Ever'body gonna be nice to you. Ain't gonna be no more trouble. Nobody gonna hurt nobody nor steal from 'em." Jury NJ, DiBerto JF, Kash TL, Holmes A. Sex differences in the behavioral sequelae of chronic ethanol exposure. Alcohol. 2017;58:53–60. Our results indicating sex differences in behavioral disinhibition during protracted abstinence led us to question whether the amount of cumulative alcohol consumption plays a role in the findings we observed here; that is, whether males would display a similar phenotype with additional alcohol drinking that achieved the cumulative alcohol consumption levels of females, or whether there are fundamental sex differences in the neural plasticity underlying these behavioral effects. Some studies report increased avoidance in males following acute abstinence from long term (8+ weeks) voluntary alcohol drinking [ 41] while others show no effect in some or all measures [ 42, 52] but to our knowledge, none have investigated these behaviors in protracted abstinence. Future work is necessary to further our understanding of the mechanism underlying our finding that post-chronic alcohol behavioral disinhibition is more pronounced in females and to dissect the role of risk-taking and compulsivity in binge drinking behavior to refine our understanding of the results in our study. Thus, there are sex differences underlying the physiological and behavioral responses to alcohol, and women may be more vulnerable to maladaptive effects that potentially drive future risky behaviors. Perspective and significance Kezer CA, Simonetto DA, Shah VH. Sex differences in alcohol consumption and alcohol-associated liver disease. Mayo Clin Proc. 2021;96(4):1006–16. Of Mice And Men' is set in the Great Depression era. The story revolves around two migrant ranch workers, George and Lennie, searching for a job as they move across America.

Sure they all want it. Everybody wants a little bit of land, not much. Jus' som'thin' that was his. Som'thin' he could live on and there couldn't nobody throw him off of it. I never had none." Themes The predatory nature of human existence; the importance of fraternity and idealized relationships between men; the impossibility of the American Dream; the destructive imbalance of social power structures in American societyMood: The novel shows a sad mood in the beginning but turns to doomed and helplessness as soon as George and Lennie move out to some other ranch and then turns to tragic when George shoots Lennie after he accidentally kills Curley’s wife. Peltier MR, Verplaetse TL, Mineur YS, Petrakis IL, Cosgrove KP, Picciotto MR, et al. Sex differences in stress-related alcohol use. Neurobiol Stress. 2019;10: 100149. Of Mice and Men tells the story of how George and Lennie’s friendship is tested by the isolating and predatory reality of life for poor migrant workers in Depression-era America. George and Lennie are the protagonists, and their friendship is unique in the world of the novella: almost every other character notes that they have never seen such a close partnership between two migrant laborers before. George and Lennie’s biggest struggle is centered around surviving their oppressive, impoverished circumstances and becoming financially stable enough to own land together. This dream of one day purchasing a farm is complicated by Lennie’s inability to stay out of trouble on the job, and George’s inability to stay angry at Lennie long enough to leave and find work on his own.

The Boss: A favorite of Candy, the Boss is the ranch owner who employs Lennie and George when they move from California to some other ranch for work. Candy’s likeness of him is due to his generosity of offering them whiskey on Christmas. Yu W, Hwa LS, Makhijani VH, Besheer J, Kash TL. Chronic inflammatory pain drives alcohol drinking in a sex-dependent manner for C57BL/6J mice. Alcohol. 2019;77:135–45.

Loneliness: The story shows the impact of loneliness and how it proves a torturing problem for a person. Lennie, due to his autistic nature, cannot live alone. Therefore, he continues to live along with George, while George also is unable to find a dependable solution or place for him. Therefore, they both try to dispel their loneliness through their friendship. Also, Curley’s wife does not see Lennie as handsome enough to cheat Curley; rather, she comes to dispel her loneliness but loses her life in her efforts to end it. Full size image Behavioral disinhibition following a history of binge alcohol drinking was especially robust in females

I was only foolin', George. I don't want no ketchup. I wouldn't eat no ketchup if it was right here beside me.'Sidhu H, Kreifeldt M, Contet C. Affective disturbances during withdrawal from chronic intermittent ethanol inhalation in C57BL/6J and DBA/2J male mice. Alcohol Clin Exp Res. 2018;42(7):1281–90. Seventy-two hour after the last alcohol/water access of eight-cycle DID (20% EtOH and H2O), mice were assessed for their preference for 1% sucrose over 3 consecutive days, followed by 2% sucrose for the subsequent 3 days, using a 24 h two-bottle choice assay as previously described [ 28]. The home cage water bottle was replaced with two 50 ml bottles, one containing 1% sucrose and one containing water, and bottles were weighed and replaced in switched positions every 24 h for 3 days. The following 3 days, the same procedure was repeated with bottles containing water and 2% sucrose. Sucrose and water intake were recorded, and consumption and preference were analyzed for days 2–3 for each sucrose concentration. Data were excluded if a bubble in the bottle spout prevented access to the sucrose on that day. Open field test