I’m trying to bring a new definition to the word ‘bimbo’, to reclaim the word in a good way,” she says. “The way it is being used today is to empower someone to be in love with themself, almost obsessed, not having to prove their smarts or who they are. Maybe you can be ditsy and bubbly and have fun and not have to prove anything to anyone. It’s getting used to being underestimated and using it to your advantage when, despite your persona, you know your own wisdom. The word doesn’t hurt. Focus on the message.” A. K. Varkouhi, M. Scholte, G. Storm and H. J. Haisma, J. Controlled Release, 2011, 151, 220–228 CrossRef CAS PubMed. E. Tasciotti, X. Liu, R. Bhavane, K. Plant, A. D. Leonard, B. K. Price, M. M. Cheng, P. Decuzzi, J. M. Tour, F. Robertson and M. Ferrari, Nat. Nanotechnol., 2008, 3, 151–157 CrossRef CAS PubMed.

H. A. Santos, L. M. Bimbo, B. Herranz, M.-A. Shahbazi, J. Hirvonen and J. Salonen, J. Mater. Res., 2013, 28, 152–164 CrossRef CAS. N. Hasan, A. Mann, M. Ferrari and T. Tanaka, Methods Mol. Biol., 2013, 1049, 481–493 Search PubMed. Fig. 2 Effect of human plasma protein adsorption on the size (A), ζ-potential (B), and PdI (C) of the UnTHCPSi and UnTHCPSi–HA + nanoparticles after incubation with human plasma for 120 min at 37 °C. The results were calculated from the DLS measurement data as a function of time. Values are represented as mean ± s.d. ( n ≥ 3). P. Kinnari, E. Mäkilä, T. Heikkilä, J. Salonen, J. Hirvonen and H. A. Santos, Int. J. Pharm., 2011, 414, 148–156 CrossRef CAS PubMed.D. Liu, L. M. Bimbo, E. Mäkilä, F. Villanova, M. Kaasalainen, B. Herranz-Blanco, C. M. Caramella, V. P. Lehto, J. Salonen, K. H. Herzig, J. Hirvonen and H. A. Santos, J. Controlled Release, 2013, 170, 268–278 CrossRef CAS PubMed. C. Wang, E. M. Mäkilä, M. H. Kaasalainen, D. Liu, M. P. Sarparanta, A. J. Airaksinen, J. J. Salonen, J. T. Hirvonen and H. A. Santos, Biomaterials, 2014, 35, 1257–1266 CrossRef CAS PubMed. M. P. Sarparanta, L. M. Bimbo, E. M. Mäkilä, J. J. Salonen, P. H. Laaksonen, A. M. Helariutta, M. B. Linder, J. T. Hirvonen, T. J. Laaksonen, H. A. Santos and A. J. Airaksinen, Biomaterials, 2012, 33, 3353–3362 CrossRef CAS PubMed.

D. Liu, H. Zhang, B. Herranz-Blanco, E. Mäkilä, V. Lehto, J. Salonen, J. Hirvonen and H. A. Santos, Small, 2014, 10, 2029–2038 CrossRef CAS PubMed.

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This will be a variety of kinks and fetishes, both existing franchises and original characters. Language: English Words: 24,433 Chapters: 5/5 Kudos: 43 Bookmarks: 14 Hits: 21,038 N2 - Substitution of fossil fuels by sustainable practices must be rapidly implemented to mitigate the impacts of climate change. The conversion of biomass into combustible gas is investigated in a microwave-induced plasma reactor using pure steam as the plasma working gas for the first time. The optimum results are achieved at the highest forward microwave power of 6 kW with biomass carbon conversion efficiency over 98% and complete biomass energy recovery in syngas. Unreacted steam is simply condensed out, leading to the production of a syngas with low inert dilution and high calorific value in the range 10.5–12 MJ/Nm3. The syngas produced is rich in hydrogen, exceeding 60% by volume. The proposed process could aid in the transition to a carbon neutral economy as it has the potential to efficiently convert biomass to syngas that can be used for the sustainable generation of fuels, chemicals and energy.

The size and morphology of the UnTHCPSi and UnTHCPSi–HA + nanoparticles were analyzed by transmission electron microscopy (TEM). The TEM pictures were captured using a Jeol JEM-1400 microscope (Jeol Ltd., Tokyo, Japan) at an 80 kV voltage. The nanoparticle suspensions were centrifuged, re-dispersed in ethanol at a concentration of 10 μg mL −1, and dropped on a carbon-coated copper TEM grid, followed by drying for 48 h at room temperature. Stability studies in human plasma For performing these experiments, 300 μg of UnTHCPSi and UnTHCPSi–HA + were dispersed in 200 μL of 1× PBS (pH 7.4), exposed to 1500 μL of human plasma, and left under stirring at 800 rpm and 37 °C for 2 h. Sample aliquots of 200 μL were withdrawn at pre-determined time points (1, 5, 10, 15, 30, 60, 90, and 120 min), and the corresponding size, ζ-potential, and PdI were subsequently determined by DLS and ELS. The results are shown as the average of at least three independent measurements. Anonymous human plasma donors were obtained from the Finnish Red Cross Blood Service, with the permission from the respective institutional ethical committee. Cell lines and culturing conditions For the in vitro studies, MCF-7 and MDA-MB-231 breast cancer cells were cultured according to the protocols described in detail in the ESI. † In vitro cytotoxicity studies The in vitro cytotoxicity of both UnTHCPSi and UnTHCPSi–HA + was evaluated by a CellTiter-Glo ® Luminescent Cell Viability assay, as previously described elsewhere. 26,29 MCF-7 and MDA-MB-231 breast cancer cells were suspended in the corresponding cell culture media at a concentration of 2 × 10 5 cells per mL, and approximately 2 × 10 4 cells per well were seeded in 96-well plates (Corning Inc. Life Sciences, USA). The cells were allowed to attach overnight at 37 °C, after which the cell culture medium was removed and replaced with 100 μL of UnTHCPSi and UnTHCPSi–HA + nanoparticle suspensions at concentrations of 25, 50, and 100 μg mL −1, with 1× HBSS (pH 7.4) and 1% Triton X-100 as positive and negative controls, respectively. After incubating for 6 and 24 h at 37 °C, 100 μL of the assay reagent was added to each well and the number of viable cells was determined by measuring the luminescence from the living cells using a Varioskan Flash fluorometer (Thermo Fisher Scientific Inc., USA). The results presented correspond to the average of at least three independent measurements. Cellular internalization The cellular uptake and intracellular localization of the UnTHCPSi and UnTHCPSi–HA + nanoparticles was assessed by TEM. Approximately 10 5 cells per well of MCF-7 and MDA-MB-231 breast cancer cells were seeded in 24-well plates (Corning Inc. Life Sciences, USA) with each well containing a 13 mm round shaped coverslip and allowed to attach overnight at 37 °C. After removing the cell culture media, 500 μL per well of the nanoparticle suspensions at a concentration of 50 μg mL −1 were added to the wells. After an incubation period of 6 h at 37 °C, the nanoparticle suspensions were carefully removed and the samples were rinsed twice with HBSS–HEPES (pH 7.4). Then, the cells were fixed with 2.5% glutaraldehyde in 0.1 M PBS buffer (pH 7.4) for 1 h at room temperature, and subsequently washed twice with HBSS–HEPES (pH 7.4) and sodium cacodylate buffer (NaCac) for 3 min, prior to post-fixation with 1% osmium tetroxide in 0.1 M NaCac buffer (pH 7.4). Thereafter, the cells were dehydrated with 30–100% ethanol for 10 min and embedded in epoxy resin. Ultrathin sections with approximately 60 nm were sliced parallel to the coverslips, post-stained with uranyl acetate and lead citrate, and finally analyzed by TEM as described above. Flow cytometric analysis of CD44 expression and cellular association The expression of CD44 receptor in MCF-7 and MDA-MB-231 breast cancer cell lines was evaluated by flow cytometry according to a protocol adapted from the manufacturer's instructions, as described in the ESI. † Fig. 4 Cell viability of intestinal HT-29 cancer cells after 3 h incubation with different concentrations (mg mL −1) of the bare THCPSi and THCPSi–lipid vesicle microparticles assessed by a luminescence-based assay. All experiments were conducted at 37 °C. Errors bars represent the mean ± SD ( n = 4). After removing the medium and washing the wells twice with fresh 1× Hanks Balanced Salt Solution pH 7.4 (HBSS), the samples were added to the wells and incubated for 3 h. For positive and negative controls, HBSS (pH 7.4; 100% cell viability) and triton X-100 (<1% cell viability) were used. Statistical analysis was made by a one-way analysis of variance (ANOVA), followed by a Dunnett's multiple comparison test to analyze the data using GraphPad Prism v. 5.01 (GraphPad Software). The level of significance was set at probability of * p< 0.05.In Y2K pop culture, almost every woman who was afforded ample screen time was impossibly attractive and in some way, a bimbo. With Legally Blonde the rare exception, the women in these movies were a bit silly at best, and at worst, morally corrupt. While I’m sure the message Tiny Fey was hoping to send when she wrote Regina George was that being a vain, popular bitch is bad and increases your chances of being hit by a bus, my eight-year-old self missed the point entirely.